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İyi seyirler. Transformasyonun amacı...","preview":{"posterSrc":"//avatars.mds.yandex.net/get-vthumb/4450512/cba7f877232f87d145617aa2dc6d381b/564x318_1","videoSrc":"https://video-preview.s3.yandex.net/k_Q1gQEAAAA.mp4","videoType":"video/mp4"},"target":"_self","position":"3","reqid":"1769490340676999-17935363012713886099-balancer-l7leveler-kubr-yp-sas-220-BAL","summary":{"isFull":true,"fullTextUrl":"/video/result?ask_summarization=1&numdoc=1&noreask=1&nomisspell=1&parent-reqid=1769490340676999-17935363012713886099-balancer-l7leveler-kubr-yp-sas-220-BAL&text=videoid:12056221761962484340","teaser":[{"list":{"type":"unordered","items":["Bu video, bakteri dönüşüm ve protein saflaştırma sürecini adım adım anlatan bir eğitim içeriğidir.","Video, bakterilerin çevrelerinden DNA plazmitleri almasına neden olan dönüşüm sürecini detaylı olarak açıklamaktadır. İlk olarak kalsiyum klorür kullanılarak hücre zarlarının yüklerinin nötralize edilmesi, ardından ısı şoku uygulanması ve son olarak antibiyotik ve indükleyici içeren plaklarda test edilmesi anlatılmaktadır. Başarılı dönüşümlerin kırmızı koloniler olarak belirlenmesi ve ardından MFP proteininin saflaştırılması için gerekli adımlar gösterilmektedir."]},"endTime":216,"title":"Bakteri Transformasyon ve Protein Saflaştırma Süreci","beginTime":0}],"fullResult":[{"index":0,"title":"Bakteri Transformasyon Süreci","list":{"type":"unordered","items":["Transformasyonun amacı, bakterilerin çevrelerinden DNA plazmitleri almalarına neden olmaktır.","Bakteriler ve plazmitler negatif yüklü olduğundan birbirlerini itme eğilimindedirler, bu etkileşimi kolaylaştırmak için kalsiyum klorür kullanılır.","Kalsiyum iyonları hücre yüzeyindeki negatif yüklü fosfat gruplarına bağlanarak yüklerini nötralize eder."]},"beginTime":5,"endTime":41,"href":"/video/preview/12056221761962484340?parent-reqid=1769490340676999-17935363012713886099-balancer-l7leveler-kubr-yp-sas-220-BAL&text=TransformaSom&t=5&ask_summarization=1"},{"index":1,"title":"Isı Şoku ve Dönüşüm","list":{"type":"unordered","items":["İlgili geni içeren plazmitler 4 santigrat derece sıcaklıkta işlenmiş hücreler ile karıştırılır, sıcaklık 42 santigrat dereceye çıkarılıp tekrar 4 santigrat dereceye düşürülür.","Isı şoku, yapışma bölgelerinin oluşmasına neden olarak sızdıran bir plazma oluşturur ve hücrenin çözeltiden bir plazmit almasını sağlar.","Isı şokundan sonra bakteri hücreleri 37 santigrat dereceye yerleştirilir ve plaklanmadan önce iyileşmeye bırakılır."]},"beginTime":41,"endTime":77,"href":"/video/preview/12056221761962484340?parent-reqid=1769490340676999-17935363012713886099-balancer-l7leveler-kubr-yp-sas-220-BAL&text=TransformaSom&t=41&ask_summarization=1"},{"index":2,"title":"Dönüşüm Testleri","list":{"type":"unordered","items":["Bakteri numuneleri başarılı dönüşümleri test etmek için agar plaklarına yerleştirilir.","Soldaki plaka tek başına antibiyotik ampisilin içerir, sağdaki plaka ise ampisilin ve şeker arabinos içerir.","Solda herhangi bir plazmit olmadan dönüşüm reaksiyonun yapıldığı negatif kontrol plakası, ortada ise 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oluşturacaktır."]},"beginTime":112,"endTime":142,"href":"/video/preview/12056221761962484340?parent-reqid=1769490340676999-17935363012713886099-balancer-l7leveler-kubr-yp-sas-220-BAL&text=TransformaSom&t=112&ask_summarization=1"},{"index":4,"title":"Protein Saflaştırılması","list":{"type":"unordered","items":["Kırmızı koloniler, mutant floresan proteini MFP'yi ifade eden ilgi konusu geni RFP içeren plazmitlere dönüştürülmüş hücreleri gösterir.","Protein saflaştırılması için MFP sentezleyen hücre popülasyonu daha da genişletmek için steril bir öze ile tek bir kırmızı koloni toplanır ve hem ampsilin hem de arabinoz içeren büyüme ortamında aşılanır.","Şişe gece boyunca çalkacıya yerleştirilir, ertesi sabah kırmızı bulutlu bir ortam oluşur, hücreler santrifüjde pelettlenir ve süpernotan 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transformasyon. Konuşmacı her bir yöntemi kendi aklına gelen örneklerle açıklamaktadır: konjugasyonu \"konjuge asit-baz çiftleri\"ne benzeterek çiftleşen bakterilerin plazmit transferi, transdüksiyonu virüs aracılığıyla gerçekleşen genetik madde transferi ve transformasyonu ise aracısız genetik madde transferi olarak anlatmaktadır."]},"endTime":226,"title":"Bakterilerde Genetik Madde Aktarımı","beginTime":0}],"fullResult":[{"index":0,"title":"Bakterilerde Genetik Madde Aktarımı","list":{"type":"unordered","items":["Bakterilerde genetik madde aktarımı, dışarıdan başka bir genetik materyalin bakterinin içine girmesi durumudur.","Genetik madde aktarımı konjugasyon, transdüksiyon ve transformasyon olmak üzere üç başlıkta incelenir."]},"beginTime":1,"endTime":22,"href":"/video/preview/5739087324963508161?parent-reqid=1769490340676999-17935363012713886099-balancer-l7leveler-kubr-yp-sas-220-BAL&text=TransformaSom&t=1&ask_summarization=1"},{"index":1,"title":"Konjugasyon","list":{"type":"unordered","items":["Konjugasyonda çift olmuş bakteriler arasında köprü kurulur ve kendi kromozomları ile plazmit adı verilen DNA parçaları arasında transfer gerçekleşir.","Plazmitler antibiyotik rezistansı gibi özelliklerin birbirlerine aktarılmasını sağlar ve bu aktarım tek yönlüdür.","Konjugasyon aynı zamanda seks köprüsü veya seks pili olarak da adlandırılır."]},"beginTime":22,"endTime":98,"href":"/video/preview/5739087324963508161?parent-reqid=1769490340676999-17935363012713886099-balancer-l7leveler-kubr-yp-sas-220-BAL&text=TransformaSom&t=22&ask_summarization=1"},{"index":2,"title":"Transdüksiyon","list":{"type":"unordered","items":["Transdüksiyonda genetik madde transferi için bir araca ihtiyaç vardır ve bu aracın 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